![]() ![]() When using deep well microplates possible leakage and subsequent cross contamination between wells should be concern. Note that steel beads can be used in common plastic vials and common deep well microvials for wet grinding, provided that the vial is completely filled with aqueous media and the amount of entrapped air is minimized. To the tubes or wells add either one 6.3 mm diameter chrome-steel bead, two 6.3 mm diameter zirconia beads or three 3.2 mm diameter chrome-steel beads. Common plastic microvials and deep well plates will break when dry grinding with steel beads. ![]() When dry- or cryo-grinding plant or animal tissue use our stainless steel microvials with silicone rubber caps, special XXTuff microvials or special Porvair Sciences deep well microplates. Most dry grinding is done using steel beads. Exceptions to the rule are woody tissue and some tissues having high lipid or oil content. The Mini-BeadBeater-96 can be used to powder most dried plant and animal tissues and, at liquid nitrogen temperatures, fresh tissues. Strict temperature control is not a needed when cells or tissue are being disrupted in the presence of DNA or RNA extraction media. For this same reason, operating a bead mill cell disruptor in a cold room offers little regulation of heat buildup during beadbeating.īut this is not always a concern. The fact is that air is not an efficient media for the transfer and rapid removal of heat. Cooled beforehand in a deep freezer or with liquid nitrogen, the mass of the aluminum vial holder and its excellent heat transfer properties maintain samples at their initial low temperature during the entire beadbeating run.something that some competitor's bead mills offering flowing cold air accessories fail to accomplish. An important accessory for the MBB-96 is a 48 capacity 2 ml microvial holder and a 2 tube capacity 50 ml tube holder, both machined out of solid aluminum. The Mini-BeadBeater-96, like all other high-energy, shaking-type bead mill cell disrupters, will heat up a sample from collisional friction of the beads.about 10° for each minute of beadbeating. ![]() Temperature control must be addressed when using a bead mill cell disrupter for the isolation of proteins, membranes or organelles. Selected Applications Using the Mini-Beadbeater A solid aluminum vial holder can be utilized to maintain cryogenic temperatures during beadbeating (see the Accessories, below). Powdered material can be safely dissolved in an organic solvent in BioSpec's XXTuff microvials, our stainless steel microvials or Porvair's reinforced microplates, simplifying recovery of organic analytes. Softer materials such as biological tissue, rubber or plastics can be powdered by first pre-freezing the sample to liq N 2 temperatures (called Cryo-grinding). In this application, ceramic or steel beads are added to to vials containing hard, dry samples such as hair, bone, teeth, seeds and minerals. The MiniBeadbeater can also be used for dry grinding. Most p rotocols developed using smaller capacity models of the MBB are transferable with little if any modification. The synergy of simultaneous mechanical cell disruption and nucleic acid extraction in a chaotropic solution gives very high yields of high quality RNA or DNA. Up to 400 mg of tissue is added to a vial containing glass or ceramic beads and a nucleic acid extraction solution.the later often being purchased as part of a nucleic acid extraction kit. A common application is nucleic acid extraction. Complete cell disruption is achieved in 1 to 3 minutes of beadbeating. The MiniBeadbeater-96 disrupts cells and tissue by violently shaking a sample inside a classic 2 ml microvial or standard deep well microplate partially filled with tiny ceramic or steel beads. ![]()
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